polyclonal primary antibody against sucnr1  (Novus Biologicals)

Journal: The Journal of Clinical Investigation

Article Title: SUCNR1 regulates insulin secretion and glucose elevates the succinate response in people with prediabetes

doi: 10.1172/JCI173214

Figure Lengend Snippet: ( A ) Sucnr1 mRNA levels analyzed in subcutaneous white adipose tissue (scWAT), visceral WAT tissue (vWAT), and liver, pancreas, and muscle tissue from male mice by quantitative PCR ( n = 3–4). ( B ) Immunohistochemical (IHC) staining of SUCNR1 in male human and male mouse pancreas sections, and chromogranin A IHC staining or H&E staining. Scale bars: 50 μm. ( C ) Analysis of Sucnr1 mRNA expression in α and β cells isolated by FACS from male rat islets ( n = 4). ( D ) In silico study of SUCNR1 gene expression regulation by genomic sequences and specific human adult islet transcriptional factors, and single-nucleotide polymorphisms (SNPs) associated with T2D localized within or surrounding the SUCNR1 locus. TFBS, transcription factor binding site. Data are presented as mean ± SEM.

Article Snippet: A polyclonal primary antibody against SUCNR1 (NBP1-0086) was obtained from Novus Biologicals.

Techniques: Real-time Polymerase Chain Reaction, Immunohistochemical staining, Immunohistochemistry, Staining, Expressing, Isolation, In Silico, Gene Expression, Genomic Sequencing, Binding Assay

Journal: The Journal of Clinical Investigation

Article Title: SUCNR1 regulates insulin secretion and glucose elevates the succinate response in people with prediabetes

doi: 10.1172/JCI173214

Figure Lengend Snippet: ( A ) Sucnr1 mRNA expression in the entire pancreas of wild-type mice fed normal chow diet (NCD) and high-fat diet (HFD) and db/db mice on NCD ( n = 4–5). ( B ) SUCNR1 and GLP1R mRNA expression in islets from healthy donors ( n = 7) and donors with obesity ( n = 3). ( C ) SUCNR1 protein levels in human islet lysates from healthy donors ( n = 6) and donors with obesity ( n = 3) and T2D ( n = 7). ( D ) Linear correlations between the BMI of donors and SUCNR1 mRNA ( n = 10) and protein ( n = 16) expression. Data are presented as mean ± SEM. * P < 0.05 vs. control (Student’s t test in A and B , Kruskal-Wallis test with Dunn’s test for multiple comparisons in C , or Pearson’s correlation coefficient in D ).

Article Snippet: A polyclonal primary antibody against SUCNR1 (NBP1-0086) was obtained from Novus Biologicals.

Techniques: Expressing, Control

Journal: The Journal of Clinical Investigation

Article Title: SUCNR1 regulates insulin secretion and glucose elevates the succinate response in people with prediabetes

doi: 10.1172/JCI173214

Figure Lengend Snippet: ( A ) Succinate quantification in the conditioned medium (CM) of MIN6 cells cultured in low- or high-glucose conditions ( n = 6). ( B ) Sucnr1 mRNA expression in MIN6 cells stimulated with different concentrations of glucose for 3 or 24 hours ( n = 4). ( C ) SUCNR1 protein levels in EndoC-βH1 cells stimulated with different concentrations of glucose for 24 hours ( n = 6). ( D ) Insulin quantification in the CM of MIN6 cells stimulated with succinate or cis -epoxysuccinic acid ( c ESA) at 2.8 mM or 16.7 mM glucose ( n = 4). ( E ) Insulin secretion in glucose-stimulated insulin secretion assays in EndoC-βH5 cells stimulated with 500 μM succinate or 50 μM c ESA at 0 mM or 20 mM glucose, determined in the CM by ELISA ( n = 4). ( F ) Insulin secretion in EndoC-βH5 cells incubated with a human-specific SUCNR1 antagonist (1 μM NF-56-EJ40) and 500 μM succinate or 50 μM c ESA ( n = 4). Data are presented as mean ± SEM. * P < 0.05, ** P < 0.01, *** P < 0.001 vs. basal conditions; ### P < 0.001 vs. succinate (Student’s t test in A , ANOVA with Dunnett’s test for multiple comparisons in B – E , or ANOVA with Tukey’s test for multiple comparisons in F ).

Article Snippet: A polyclonal primary antibody against SUCNR1 (NBP1-0086) was obtained from Novus Biologicals.

Techniques: Cell Culture, Expressing, Enzyme-linked Immunosorbent Assay, Incubation

Journal: The Journal of Clinical Investigation

Article Title: SUCNR1 regulates insulin secretion and glucose elevates the succinate response in people with prediabetes

doi: 10.1172/JCI173214

Figure Lengend Snippet: ( A ) IHC staining of SUCNR1 in pancreas sections of control and Sucnr1 -βKO mice accompanied by serial H&E staining. Scale bars: 50 μm. ( B ) Body mass of control and Sucnr1 -βKO mice under HFD for 8 weeks ( n = 8–9). ( C ) Blood glucose levels in control and Sucnr1 -βKO mice in fasted or random-fed conditions ( n = 8). ( D ) Plasma insulin levels in control and Sucnr1 -βKO mice in fasted or random-fed conditions ( n = 7–8). ( E ) Morphometric analysis of control and Sucnr1 -βKO mice by H&E staining ( n = 3). Scale bars: 50 μm. ( F ) Morphometric analysis of control and Sucnr1 -βKO mice by immunofluorescence staining with insulin and glucagon and counterstaining with DAPI ( n = 3–4). Scale bars: 50 μm. ( G ) Intraperitoneal (i.p.) and oral glucose tolerance tests in control and Sucnr1 -βKO mice ( n = 6–7). Displayed are the blood glucose levels, AUC, plasma insulin ( n = 5–6), and GLP-1 levels ( n = 5). ( H ) Insulin tolerance test in control and Sucnr1 -βKO mice ( n = 6–8). ( I ) HOMA-IR for control and Sucnr1 -βKO mice ( n = 5). ( J ) Insulin secretion in isolated islets from control and Sucnr1 -βKO mice stimulated with or without 1 mM succinate or 100 μM c ESA at 2.8 or 16.7 mM glucose ( n = 5 islet pools from 5–6 mice). Data are presented as mean ± SEM or as box-and-whisker plots indicating median, first and third quartiles, and maximum and minimum values. * P < 0.05, ** P < 0.01, *** P < 0.001 vs. control mice, comparing experimental groups in orally administered mice, or in indicated pairwise comparisons; ## P < 0.01, ### P < 0.001 comparing experimental groups in i.p.-administered mice (Student’s t test in B , E , F , and I comparing 2 groups, or 2-way ANOVA with Bonferroni’s test for multiple comparisons in C , D , G , H , and J ).

Article Snippet: A polyclonal primary antibody against SUCNR1 (NBP1-0086) was obtained from Novus Biologicals.

Techniques: Immunohistochemistry, Control, Staining, Clinical Proteomics, Immunofluorescence, Isolation, Whisker Assay